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act a peprotech  (PeproTech)
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PeproTech act a peprotech
Act A Peprotech, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/act+a+peprotech/pm37106915-100-15-31?v=PeproTech
Average 90 stars, based on 1 article reviews
act a peprotech - by Bioz Stars, 2026-07
90/100 stars
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act a  (PeproTech)
90
PeproTech act a
Study design of the <t>cardiomyogenic</t> differentiation experiments using the induction factors activin A (Act A), bone morphogenetic protein-4 (BMP-4) and Dickkopf-1 (DKK-1). In both sub-experiments, equine adipose-derived stem cells were induced with Act A on day 0, with BMP-4 from day 1 to 5 and with DKK-1 from day 5 to 7 in laminin-coated cell culture flasks. Further cultivation from day 7 on was performed in a modified basal medium, and, on day 21, cells were harvested for subsequent molecular–biological studies. Abbreviations: DMEM, Dulbecco’s Modified Eagle’s Medium; FBS, fetal bovine serum; MC, medium change; T0, time point day 0; T3, time point 3 weeks after induction.
Act A, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/act+a+peprotech/pmc10135324-100-8-32?v=PeproTech
Average 90 stars, based on 1 article reviews
act a - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

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Study design of the cardiomyogenic differentiation experiments using the induction factors activin A (Act A), bone morphogenetic protein-4 (BMP-4) and Dickkopf-1 (DKK-1). In both sub-experiments, equine adipose-derived stem cells were induced with Act A on day 0, with BMP-4 from day 1 to 5 and with DKK-1 from day 5 to 7 in laminin-coated cell culture flasks. Further cultivation from day 7 on was performed in a modified basal medium, and, on day 21, cells were harvested for subsequent molecular–biological studies. Abbreviations: DMEM, Dulbecco’s Modified Eagle’s Medium; FBS, fetal bovine serum; MC, medium change; T0, time point day 0; T3, time point 3 weeks after induction.

Journal: Animals : an Open Access Journal from MDPI

Article Title: Multilineage Differentiation Potential of Equine Adipose-Derived Stromal/Stem Cells from Different Sources

doi: 10.3390/ani13081352

Figure Lengend Snippet: Study design of the cardiomyogenic differentiation experiments using the induction factors activin A (Act A), bone morphogenetic protein-4 (BMP-4) and Dickkopf-1 (DKK-1). In both sub-experiments, equine adipose-derived stem cells were induced with Act A on day 0, with BMP-4 from day 1 to 5 and with DKK-1 from day 5 to 7 in laminin-coated cell culture flasks. Further cultivation from day 7 on was performed in a modified basal medium, and, on day 21, cells were harvested for subsequent molecular–biological studies. Abbreviations: DMEM, Dulbecco’s Modified Eagle’s Medium; FBS, fetal bovine serum; MC, medium change; T0, time point day 0; T3, time point 3 weeks after induction.

Article Snippet: Based on our previous study, in which no cardiomyogenic differentiation was achieved using 5-AZA in uncoated cell culture flasks [ ], the cardiomyogenic differentiation protocol of the present study applied Act A (Peprotech, Hamburg, Germany) for 24 h, BMP-4 (R&D Systems, Inc., Minneapolis, MN, USA) for 96 h and DKK-1 (Peprotech, Hamburg, Germany) in abd-ASCs- SVF (P3–6) for 48 h ( n = 5), factors that are involved in embryonic cardiomyogenesis [ , , , , , ].

Techniques: Derivative Assay, Cell Culture, Modification

Application of substances for cardiomyogenic differentiation. Cell morphology of equine adipose-derived stem cells exposed to different concentrations of activin (Act) A, bone morphogenetic protein (BMP)-4 and Dickkopf (DKK)-1 in ( A ) a first and ( B ) a second sub-experiment. Cell morphology of two representative horses is shown before (day 0) and 21 days after cardiomyogenic induction. Neither relevant differences between the induced cells and non-induced negative controls nor a cardiomyogenic differentiation could be detected. Cells retained their fibroblast-like cell morphology and formed “domes”, which were more obvious in horse 16 compared to horse 15, particularly three weeks after induction. Scale bar: 100 µm (10× objective).

Journal: Animals : an Open Access Journal from MDPI

Article Title: Multilineage Differentiation Potential of Equine Adipose-Derived Stromal/Stem Cells from Different Sources

doi: 10.3390/ani13081352

Figure Lengend Snippet: Application of substances for cardiomyogenic differentiation. Cell morphology of equine adipose-derived stem cells exposed to different concentrations of activin (Act) A, bone morphogenetic protein (BMP)-4 and Dickkopf (DKK)-1 in ( A ) a first and ( B ) a second sub-experiment. Cell morphology of two representative horses is shown before (day 0) and 21 days after cardiomyogenic induction. Neither relevant differences between the induced cells and non-induced negative controls nor a cardiomyogenic differentiation could be detected. Cells retained their fibroblast-like cell morphology and formed “domes”, which were more obvious in horse 16 compared to horse 15, particularly three weeks after induction. Scale bar: 100 µm (10× objective).

Article Snippet: Based on our previous study, in which no cardiomyogenic differentiation was achieved using 5-AZA in uncoated cell culture flasks [ ], the cardiomyogenic differentiation protocol of the present study applied Act A (Peprotech, Hamburg, Germany) for 24 h, BMP-4 (R&D Systems, Inc., Minneapolis, MN, USA) for 96 h and DKK-1 (Peprotech, Hamburg, Germany) in abd-ASCs- SVF (P3–6) for 48 h ( n = 5), factors that are involved in embryonic cardiomyogenesis [ , , , , , ].

Techniques: Derivative Assay